diff --git a/pandas/conftest.py b/pandas/conftest.py
index 7bfd1b35f5314..2d8f9442d4e76 100644
--- a/pandas/conftest.py
+++ b/pandas/conftest.py
@@ -188,8 +188,8 @@ def pytest_collection_modifyitems(items, config) -> None:
         ("read_parquet", "Passing a BlockManager to DataFrame is deprecated"),
     ]
 
-    for item in items:
-        if is_doctest:
+    if is_doctest:
+        for item in items:
             # autouse=True for the add_doctest_imports can lead to expensive teardowns
             # since doctest_namespace is a session fixture
             item.add_marker(pytest.mark.usefixtures("add_doctest_imports"))
@@ -197,10 +197,6 @@ def pytest_collection_modifyitems(items, config) -> None:
             for path, message in ignored_doctest_warnings:
                 ignore_doctest_warning(item, path, message)
 
-        # mark all tests in the pandas/tests/frame directory with "arraymanager"
-        if "/frame/" in item.nodeid:
-            item.add_marker(pytest.mark.arraymanager)
-
 
 hypothesis_health_checks = [hypothesis.HealthCheck.too_slow]
 if Version(hypothesis.__version__) >= Version("6.83.2"):
diff --git a/pyproject.toml b/pyproject.toml
index f6ba25f448540..7b7332108815f 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -523,7 +523,6 @@ markers = [
   "db: tests requiring a database (mysql or postgres)",
   "clipboard: mark a pd.read_clipboard test",
   "arm_slow: mark a test as slow for arm64 architecture",
-  "arraymanager: mark a test to run with ArrayManager enabled",
 ]
 
 [tool.mypy]
diff --git a/scripts/tests/data/deps_minimum.toml b/scripts/tests/data/deps_minimum.toml
index 046dccb470daa..f8535635e12ab 100644
--- a/scripts/tests/data/deps_minimum.toml
+++ b/scripts/tests/data/deps_minimum.toml
@@ -382,7 +382,6 @@ markers = [
   "db: tests requiring a database (mysql or postgres)",
   "clipboard: mark a pd.read_clipboard test",
   "arm_slow: mark a test as slow for arm64 architecture",
-  "arraymanager: mark a test to run with ArrayManager enabled",
 ]
 
 [tool.mypy]