diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index 220465a1d2847..4379220c33687 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -339,6 +339,11 @@ repos:
         language: python
         entry: python scripts/validate_unwanted_patterns.py --validation-type="strings_with_wrong_placed_whitespace"
         types_or: [python, cython]
+    -   id: unwanted-patterns-nodefault-used-not-only-for-typing
+        name: Check that `pandas._libs.lib.NoDefault` is used only for typing
+        language: python
+        entry: python scripts/validate_unwanted_patterns.py --validation-type="nodefault_used_not_only_for_typing"
+        types: [python]
     -   id: use-pd_array-in-core
         name: Import pandas.array as pd_array in core
         language: python
diff --git a/scripts/tests/test_validate_unwanted_patterns.py b/scripts/tests/test_validate_unwanted_patterns.py
index 90eca13b21628..b4423197e2573 100644
--- a/scripts/tests/test_validate_unwanted_patterns.py
+++ b/scripts/tests/test_validate_unwanted_patterns.py
@@ -375,3 +375,72 @@ def test_strings_with_wrong_placed_whitespace_raises(self, data, expected):
             validate_unwanted_patterns.strings_with_wrong_placed_whitespace(fd)
         )
         assert result == expected
+
+
+class TestNoDefaultUsedNotOnlyForTyping:
+    @pytest.mark.parametrize(
+        "data",
+        [
+            (
+                """
+def f(
+    a: int | NoDefault,
+    b: float | lib.NoDefault = 0.1,
+    c: pandas._libs.lib.NoDefault = lib.no_default,
+) -> lib.NoDefault | None:
+    pass
+"""
+            ),
+            (
+                """
+# var = lib.NoDefault
+# the above is incorrect
+a: NoDefault | int
+b: lib.NoDefault = lib.no_default
+"""
+            ),
+        ],
+    )
+    def test_nodefault_used_not_only_for_typing(self, data):
+        fd = io.StringIO(data.strip())
+        result = list(validate_unwanted_patterns.nodefault_used_not_only_for_typing(fd))
+        assert result == []
+
+    @pytest.mark.parametrize(
+        "data, expected",
+        [
+            (
+                (
+                    """
+def f(
+    a = lib.NoDefault,
+    b: Any
+        = pandas._libs.lib.NoDefault,
+):
+    pass
+"""
+                ),
+                [
+                    (2, "NoDefault is used not only for typing"),
+                    (4, "NoDefault is used not only for typing"),
+                ],
+            ),
+            (
+                (
+                    """
+a: Any = lib.NoDefault
+if a is NoDefault:
+    pass
+"""
+                ),
+                [
+                    (1, "NoDefault is used not only for typing"),
+                    (2, "NoDefault is used not only for typing"),
+                ],
+            ),
+        ],
+    )
+    def test_nodefault_used_not_only_for_typing_raises(self, data, expected):
+        fd = io.StringIO(data.strip())
+        result = list(validate_unwanted_patterns.nodefault_used_not_only_for_typing(fd))
+        assert result == expected
diff --git a/scripts/validate_unwanted_patterns.py b/scripts/validate_unwanted_patterns.py
index e171d1825ac48..cffae7d18bee1 100755
--- a/scripts/validate_unwanted_patterns.py
+++ b/scripts/validate_unwanted_patterns.py
@@ -353,6 +353,52 @@ def has_wrong_whitespace(first_line: str, second_line: str) -> bool:
                 )
 
 
+def nodefault_used_not_only_for_typing(file_obj: IO[str]) -> Iterable[Tuple[int, str]]:
+    """Test case where pandas._libs.lib.NoDefault is not used for typing.
+
+    Parameters
+    ----------
+    file_obj : IO
+        File-like object containing the Python code to validate.
+
+    Yields
+    ------
+    line_number : int
+        Line number of misused lib.NoDefault.
+    msg : str
+        Explanation of the error.
+    """
+    contents = file_obj.read()
+    tree = ast.parse(contents)
+    in_annotation = False
+    nodes: List[tuple[bool, ast.AST]] = [(in_annotation, tree)]
+
+    while nodes:
+        in_annotation, node = nodes.pop()
+        if not in_annotation and (
+            isinstance(node, ast.Name)  # Case `NoDefault`
+            and node.id == "NoDefault"
+            or isinstance(node, ast.Attribute)  # Cases e.g. `lib.NoDefault`
+            and node.attr == "NoDefault"
+        ):
+            yield (node.lineno, "NoDefault is used not only for typing")
+
+        # This part is adapted from
+        # https://github.com/asottile/pyupgrade/blob/5495a248f2165941c5d3b82ac3226ba7ad1fa59d/pyupgrade/_data.py#L70-L113
+        for name in reversed(node._fields):
+            value = getattr(node, name)
+            if name in {"annotation", "returns"}:
+                next_in_annotation = True
+            else:
+                next_in_annotation = in_annotation
+            if isinstance(value, ast.AST):
+                nodes.append((next_in_annotation, value))
+            elif isinstance(value, list):
+                for value in reversed(value):
+                    if isinstance(value, ast.AST):
+                        nodes.append((next_in_annotation, value))
+
+
 def main(
     function: Callable[[IO[str]], Iterable[Tuple[int, str]]],
     source_path: str,
@@ -405,6 +451,7 @@ def main(
         "private_function_across_module",
         "private_import_across_module",
         "strings_with_wrong_placed_whitespace",
+        "nodefault_used_not_only_for_typing",
     ]
 
     parser = argparse.ArgumentParser(description="Unwanted patterns checker.")
@@ -413,7 +460,7 @@ def main(
     parser.add_argument(
         "--format",
         "-f",
-        default="{source_path}:{line_number}:{msg}",
+        default="{source_path}:{line_number}: {msg}",
         help="Output format of the error message.",
     )
     parser.add_argument(