diff --git a/doc/source/whatsnew/v1.5.0.rst b/doc/source/whatsnew/v1.5.0.rst
index 73dc832e2007b..ebbdb99bf81d2 100644
--- a/doc/source/whatsnew/v1.5.0.rst
+++ b/doc/source/whatsnew/v1.5.0.rst
@@ -601,6 +601,7 @@ Reshaping
 - Bug in :meth:`DataFrame.align` when aligning a :class:`MultiIndex` to a :class:`Series` with another :class:`MultiIndex` (:issue:`46001`)
 - Bug in concanenation with ``IntegerDtype``, or ``FloatingDtype`` arrays where the resulting dtype did not mirror the behavior of the non-nullable dtypes (:issue:`46379`)
 - Bug in :func:`concat` with identical key leads to error when indexing :class:`MultiIndex` (:issue:`46519`)
+- Bug in :meth:`DataFrame.join` with a list when using suffixes to join DataFrames with duplicate column names (:issue:`46396`)
 -
 
 Sparse
diff --git a/pandas/core/frame.py b/pandas/core/frame.py
index 683674d8ef826..1f083cbd3a71d 100644
--- a/pandas/core/frame.py
+++ b/pandas/core/frame.py
@@ -9576,6 +9576,11 @@ def _join_compat(
                     "Joining multiple DataFrames only supported for joining on index"
                 )
 
+            if rsuffix or lsuffix:
+                raise ValueError(
+                    "Suffixes not supported when joining multiple DataFrames"
+                )
+
             frames = [self] + list(other)
 
             can_concat = all(df.index.is_unique for df in frames)
diff --git a/pandas/tests/frame/methods/test_join.py b/pandas/tests/frame/methods/test_join.py
index c6bfd94b84908..36f3d04a7b6ac 100644
--- a/pandas/tests/frame/methods/test_join.py
+++ b/pandas/tests/frame/methods/test_join.py
@@ -82,6 +82,28 @@ def test_join(left, right, how, sort, expected):
     tm.assert_frame_equal(result, expected)
 
 
+def test_suffix_on_list_join():
+    first = DataFrame({"key": [1, 2, 3, 4, 5]})
+    second = DataFrame({"key": [1, 8, 3, 2, 5], "v1": [1, 2, 3, 4, 5]})
+    third = DataFrame({"keys": [5, 2, 3, 4, 1], "v2": [1, 2, 3, 4, 5]})
+
+    # check proper errors are raised
+    msg = "Suffixes not supported when joining multiple DataFrames"
+    with pytest.raises(ValueError, match=msg):
+        first.join([second], lsuffix="y")
+    with pytest.raises(ValueError, match=msg):
+        first.join([second, third], rsuffix="x")
+    with pytest.raises(ValueError, match=msg):
+        first.join([second, third], lsuffix="y", rsuffix="x")
+    with pytest.raises(ValueError, match="Indexes have overlapping values"):
+        first.join([second, third])
+
+    # no errors should be raised
+    arr_joined = first.join([third])
+    norm_joined = first.join(third)
+    tm.assert_frame_equal(arr_joined, norm_joined)
+
+
 def test_join_index(float_frame):
     # left / right