diff --git a/doc/source/whatsnew/v1.5.0.rst b/doc/source/whatsnew/v1.5.0.rst
index 358d9447b131d..6bb86a90e487a 100644
--- a/doc/source/whatsnew/v1.5.0.rst
+++ b/doc/source/whatsnew/v1.5.0.rst
@@ -569,6 +569,7 @@ I/O
 - Bug in Parquet roundtrip for Interval dtype with ``datetime64[ns]`` subtype (:issue:`45881`)
 - Bug in :func:`read_excel` when reading a ``.ods`` file with newlines between xml elements (:issue:`45598`)
 - Bug in :func:`read_parquet` when ``engine="fastparquet"`` where the file was not closed on error (:issue:`46555`)
+- Bug in :func:`read_excel` when reading a ``.xlsx`` file with a boolean column that contains blank lines (:issue:`45903`)
 
 Period
 ^^^^^^
diff --git a/pandas/core/arrays/boolean.py b/pandas/core/arrays/boolean.py
index 35b9de3f7af93..f7f276f5ed330 100644
--- a/pandas/core/arrays/boolean.py
+++ b/pandas/core/arrays/boolean.py
@@ -289,6 +289,7 @@ class BooleanArray(BaseMaskedArray):
     # Fill values used for any/all
     _truthy_value = True
     _falsey_value = False
+    _NONE_VALUES = {"nan", "NaN", "None", "NA", "null", "NULL"}
     _TRUE_VALUES = {"True", "TRUE", "true", "1", "1.0"}
     _FALSE_VALUES = {"False", "FALSE", "false", "0", "0.0"}
 
@@ -321,8 +322,8 @@ def _from_sequence_of_strings(
         false_values_union = cls._FALSE_VALUES.union(false_values or [])
 
         def map_string(s):
-            if isna(s):
-                return s
+            if isna(s) or s in cls._NONE_VALUES:
+                return None
             elif s in true_values_union:
                 return True
             elif s in false_values_union:
diff --git a/pandas/io/parsers/base_parser.py b/pandas/io/parsers/base_parser.py
index 2851ea36c8a33..b0562b06e9fea 100644
--- a/pandas/io/parsers/base_parser.py
+++ b/pandas/io/parsers/base_parser.py
@@ -785,7 +785,7 @@ def _cast_types(self, values, cast_type, column):
             try:
                 if is_bool_dtype(cast_type):
                     return array_type._from_sequence_of_strings(
-                        values,
+                        values.astype(str),
                         dtype=cast_type,
                         true_values=self.true_values,
                         false_values=self.false_values,
diff --git a/pandas/tests/io/data/excel/test_types.ods b/pandas/tests/io/data/excel/test_types.ods
index c9a82bfff810b..881c89c1f42db 100644
Binary files a/pandas/tests/io/data/excel/test_types.ods and b/pandas/tests/io/data/excel/test_types.ods differ
diff --git a/pandas/tests/io/data/excel/test_types.xls b/pandas/tests/io/data/excel/test_types.xls
index 2d387603a8307..bae1dc4815dad 100644
Binary files a/pandas/tests/io/data/excel/test_types.xls and b/pandas/tests/io/data/excel/test_types.xls differ
diff --git a/pandas/tests/io/data/excel/test_types.xlsb b/pandas/tests/io/data/excel/test_types.xlsb
index e7403aa288263..9c5ecddb7dff7 100644
Binary files a/pandas/tests/io/data/excel/test_types.xlsb and b/pandas/tests/io/data/excel/test_types.xlsb differ
diff --git a/pandas/tests/io/data/excel/test_types.xlsm b/pandas/tests/io/data/excel/test_types.xlsm
index 4c8c10e40effe..0da5807c66b7e 100644
Binary files a/pandas/tests/io/data/excel/test_types.xlsm and b/pandas/tests/io/data/excel/test_types.xlsm differ
diff --git a/pandas/tests/io/data/excel/test_types.xlsx b/pandas/tests/io/data/excel/test_types.xlsx
index 561bb2c5d6714..b6f3ae43b8060 100644
Binary files a/pandas/tests/io/data/excel/test_types.xlsx and b/pandas/tests/io/data/excel/test_types.xlsx differ
diff --git a/pandas/tests/io/excel/test_readers.py b/pandas/tests/io/excel/test_readers.py
index 1e0f74ea41453..2600f674fc6e7 100644
--- a/pandas/tests/io/excel/test_readers.py
+++ b/pandas/tests/io/excel/test_readers.py
@@ -420,8 +420,10 @@ def test_reader_special_dtypes(self, request, read_ext):
                     datetime(2013, 12, 14),
                     datetime(2015, 3, 14),
                 ],
+                "BoolColWithBlank": [True, False, None, True, False],
             },
         )
+
         basename = "test_types"
 
         # should read in correctly and infer types