diff --git a/doc/source/whatsnew/v1.5.0.rst b/doc/source/whatsnew/v1.5.0.rst
index 1ae76984484af..3a8bbf420da1a 100644
--- a/doc/source/whatsnew/v1.5.0.rst
+++ b/doc/source/whatsnew/v1.5.0.rst
@@ -227,7 +227,7 @@ MultiIndex
 I/O
 ^^^
 - Bug in :meth:`DataFrame.to_stata` where no error is raised if the :class:`DataFrame` contains ``-np.inf`` (:issue:`45350`)
--
+- Bug in :meth:`DataFrame.info` where a new line at the end of the output is omitted when called on an empty :class:`DataFrame` (:issue:`45494`)
 
 Period
 ^^^^^^
diff --git a/pandas/io/formats/info.py b/pandas/io/formats/info.py
index 504b2d632860f..dbdb1b1b96984 100644
--- a/pandas/io/formats/info.py
+++ b/pandas/io/formats/info.py
@@ -812,7 +812,7 @@ def _fill_empty_info(self) -> None:
         """Add lines to the info table, pertaining to empty dataframe."""
         self.add_object_type_line()
         self.add_index_range_line()
-        self._lines.append(f"Empty {type(self.data).__name__}")
+        self._lines.append(f"Empty {type(self.data).__name__}\n")
 
     @abstractmethod
     def _fill_non_empty_info(self) -> None:
diff --git a/pandas/tests/io/formats/test_info.py b/pandas/tests/io/formats/test_info.py
index 5522631d222e1..6bcf971e5bb05 100644
--- a/pandas/tests/io/formats/test_info.py
+++ b/pandas/tests/io/formats/test_info.py
@@ -29,6 +29,7 @@ def duplicate_columns_frame():
 
 
 def test_info_empty():
+    # GH #45494
     df = DataFrame()
     buf = StringIO()
     df.info(buf=buf)
@@ -37,7 +38,7 @@ def test_info_empty():
         """\
         <class 'pandas.core.frame.DataFrame'>
         Index: 0 entries
-        Empty DataFrame"""
+        Empty DataFrame\n"""
     )
     assert result == expected