diff --git a/doc/source/whatsnew/v0.25.1.rst b/doc/source/whatsnew/v0.25.1.rst
index 943a6adb7944e..7d78a8fe6dd84 100644
--- a/doc/source/whatsnew/v0.25.1.rst
+++ b/doc/source/whatsnew/v0.25.1.rst
@@ -103,7 +103,7 @@ MultiIndex
 I/O
 ^^^
 
--
+- Avoid calling ``S3File.s3`` when reading parquet, as this was removed in s3fs version 0.3.0 (:issue:`27756`)
 -
 -
 
diff --git a/pandas/io/parquet.py b/pandas/io/parquet.py
index 82c460300582b..6fc70e9f4a737 100644
--- a/pandas/io/parquet.py
+++ b/pandas/io/parquet.py
@@ -184,12 +184,14 @@ def write(
 
     def read(self, path, columns=None, **kwargs):
         if is_s3_url(path):
+            from pandas.io.s3 import get_file_and_filesystem
+
             # When path is s3:// an S3File is returned.
             # We need to retain the original path(str) while also
             # pass the S3File().open function to fsatparquet impl.
-            s3, _, _, should_close = get_filepath_or_buffer(path)
+            s3, filesystem = get_file_and_filesystem(path)
             try:
-                parquet_file = self.api.ParquetFile(path, open_with=s3.s3.open)
+                parquet_file = self.api.ParquetFile(path, open_with=filesystem.open)
             finally:
                 s3.close()
         else:
diff --git a/pandas/io/s3.py b/pandas/io/s3.py
index 0a7c082fec51c..7e0a37e8cba20 100644
--- a/pandas/io/s3.py
+++ b/pandas/io/s3.py
@@ -1,8 +1,11 @@
 """ s3 support for remote file interactivity """
+from typing import IO, Any, Optional, Tuple
 from urllib.parse import urlparse as parse_url
 
 from pandas.compat._optional import import_optional_dependency
 
+from pandas._typing import FilePathOrBuffer
+
 s3fs = import_optional_dependency(
     "s3fs", extra="The s3fs package is required to handle s3 files."
 )
@@ -14,9 +17,9 @@ def _strip_schema(url):
     return result.netloc + result.path
 
 
-def get_filepath_or_buffer(
-    filepath_or_buffer, encoding=None, compression=None, mode=None
-):
+def get_file_and_filesystem(
+    filepath_or_buffer: FilePathOrBuffer, mode: Optional[str] = None
+) -> Tuple[IO, Any]:
     from botocore.exceptions import NoCredentialsError
 
     if mode is None:
@@ -24,7 +27,7 @@ def get_filepath_or_buffer(
 
     fs = s3fs.S3FileSystem(anon=False)
     try:
-        filepath_or_buffer = fs.open(_strip_schema(filepath_or_buffer), mode)
+        file = fs.open(_strip_schema(filepath_or_buffer), mode)
     except (FileNotFoundError, NoCredentialsError):
         # boto3 has troubles when trying to access a public file
         # when credentialed...
@@ -33,5 +36,15 @@ def get_filepath_or_buffer(
         # A NoCredentialsError is raised if you don't have creds
         # for that bucket.
         fs = s3fs.S3FileSystem(anon=True)
-        filepath_or_buffer = fs.open(_strip_schema(filepath_or_buffer), mode)
-    return filepath_or_buffer, None, compression, True
+        file = fs.open(_strip_schema(filepath_or_buffer), mode)
+    return file, fs
+
+
+def get_filepath_or_buffer(
+    filepath_or_buffer: FilePathOrBuffer,
+    encoding: Optional[str] = None,
+    compression: Optional[str] = None,
+    mode: Optional[str] = None,
+) -> Tuple[IO, Optional[str], Optional[str], bool]:
+    file, _fs = get_file_and_filesystem(filepath_or_buffer, mode=mode)
+    return file, None, compression, True