diff --git a/ci/deps/azure-35-compat.yaml b/ci/deps/azure-35-compat.yaml
index e55a4fbdf3fa9..6643f804eb6f7 100644
--- a/ci/deps/azure-35-compat.yaml
+++ b/ci/deps/azure-35-compat.yaml
@@ -22,7 +22,6 @@ dependencies:
   - hypothesis>=3.58.0
   - pytest-xdist
   - pytest-mock
-  - isort
   - pip
   - pip:
     # for python 3.5, pytest>=4.0.2 is not available in conda
diff --git a/ci/deps/azure-36-locale.yaml b/ci/deps/azure-36-locale.yaml
index f43ed0249985e..320839756befb 100644
--- a/ci/deps/azure-36-locale.yaml
+++ b/ci/deps/azure-36-locale.yaml
@@ -24,7 +24,6 @@ dependencies:
   - pytest-xdist
   - pytest-mock
   - hypothesis>=3.58.0
-  - isort
   - pip
   - pip:
     - html5lib==1.0b2
diff --git a/ci/deps/azure-36-locale_slow.yaml b/ci/deps/azure-36-locale_slow.yaml
index 2a0404614dcfc..9ddc782da930e 100644
--- a/ci/deps/azure-36-locale_slow.yaml
+++ b/ci/deps/azure-36-locale_slow.yaml
@@ -30,7 +30,6 @@ dependencies:
   - pytest-xdist
   - pytest-mock
   - moto
-  - isort
   - pip
   - pip:
     - hypothesis>=3.58.0
diff --git a/ci/deps/azure-37-locale.yaml b/ci/deps/azure-37-locale.yaml
index 649f5892f174d..2ebb7dda86e36 100644
--- a/ci/deps/azure-37-locale.yaml
+++ b/ci/deps/azure-37-locale.yaml
@@ -28,7 +28,6 @@ dependencies:
   - pytest>=4.0.2
   - pytest-xdist
   - pytest-mock
-  - isort
   - pip
   - pip:
     - hypothesis>=3.58.0
diff --git a/ci/deps/azure-37-numpydev.yaml b/ci/deps/azure-37-numpydev.yaml
index 6848b9990b46e..831f13fb421f0 100644
--- a/ci/deps/azure-37-numpydev.yaml
+++ b/ci/deps/azure-37-numpydev.yaml
@@ -10,7 +10,6 @@ dependencies:
   - pytest-xdist
   - pytest-mock
   - hypothesis>=3.58.0
-  - isort
   - pip
   - pip:
     - "git+git://github.com/dateutil/dateutil.git"
diff --git a/ci/deps/azure-macos-35.yaml b/ci/deps/azure-macos-35.yaml
index 00c2051f29760..30290277fd644 100644
--- a/ci/deps/azure-macos-35.yaml
+++ b/ci/deps/azure-macos-35.yaml
@@ -21,7 +21,6 @@ dependencies:
   - xlrd
   - xlsxwriter
   - xlwt
-  - isort
   - pip:
     - python-dateutil==2.5.3
     # universal
diff --git a/ci/deps/azure-windows-36.yaml b/ci/deps/azure-windows-36.yaml
index 7b3ae259fb8dd..b1795059091b9 100644
--- a/ci/deps/azure-windows-36.yaml
+++ b/ci/deps/azure-windows-36.yaml
@@ -27,4 +27,3 @@ dependencies:
   - pytest-xdist
   - pytest-mock
   - hypothesis>=3.58.0
-  - isort
diff --git a/ci/deps/azure-windows-37.yaml b/ci/deps/azure-windows-37.yaml
index 5384e794d442a..04e4f74f85e4d 100644
--- a/ci/deps/azure-windows-37.yaml
+++ b/ci/deps/azure-windows-37.yaml
@@ -30,4 +30,3 @@ dependencies:
   - pytest-mock
   - moto
   - hypothesis>=3.58.0
-  - isort
diff --git a/ci/deps/travis-36-cov.yaml b/ci/deps/travis-36-cov.yaml
index 606b5bac8306d..f4eb37b4c62e4 100644
--- a/ci/deps/travis-36-cov.yaml
+++ b/ci/deps/travis-36-cov.yaml
@@ -41,7 +41,6 @@ dependencies:
   - pytest-cov
   - pytest-mock
   - hypothesis>=3.58.0
-  - isort
   - pip
   - pip:
     - brotlipy
diff --git a/ci/deps/travis-36-locale.yaml b/ci/deps/travis-36-locale.yaml
index 1d219bbcb671c..badf4e6932da8 100644
--- a/ci/deps/travis-36-locale.yaml
+++ b/ci/deps/travis-36-locale.yaml
@@ -39,7 +39,6 @@ dependencies:
   - pytest-xdist
   - pytest-mock
   - moto
-  - isort
   - pip
   - pip:
     - hypothesis>=3.58.0
diff --git a/ci/deps/travis-36-slow.yaml b/ci/deps/travis-36-slow.yaml
index 365c78c02f4d4..87021d5dae04e 100644
--- a/ci/deps/travis-36-slow.yaml
+++ b/ci/deps/travis-36-slow.yaml
@@ -30,4 +30,3 @@ dependencies:
   - pytest-mock
   - moto
   - hypothesis>=3.58.0
-  - isort
diff --git a/ci/deps/travis-37.yaml b/ci/deps/travis-37.yaml
index 4bf7d516711f7..722a35111ab01 100644
--- a/ci/deps/travis-37.yaml
+++ b/ci/deps/travis-37.yaml
@@ -18,7 +18,6 @@ dependencies:
   - pytest-mock
   - hypothesis>=3.58.0
   - s3fs
-  - isort
   - pip
   - pip:
     - moto