diff --git a/doc/source/install.rst b/doc/source/install.rst
index b3b5945cc515e..98443ede2e965 100644
--- a/doc/source/install.rst
+++ b/doc/source/install.rst
@@ -281,7 +281,6 @@ Optional Dependencies
   `qtpy  <https://github.com/spyder-ide/qtpy>`__ (requires PyQt or PySide),
   `PyQt5 <https://www.riverbankcomputing.com/software/pyqt/download5>`__,
   `PyQt4 <http://www.riverbankcomputing.com/software/pyqt/download>`__,
-  `pygtk <http://www.pygtk.org/>`__,
   `xsel <http://www.vergenet.net/~conrad/software/xsel/>`__, or
   `xclip <https://github.com/astrand/xclip/>`__: necessary to use
   :func:`~pandas.read_clipboard`. Most package managers on Linux distributions will have ``xclip`` and/or ``xsel`` immediately available for installation.
diff --git a/doc/source/user_guide/io.rst b/doc/source/user_guide/io.rst
index 88d8ccbbe036e..4aacb6fa1e278 100644
--- a/doc/source/user_guide/io.rst
+++ b/doc/source/user_guide/io.rst
@@ -3272,7 +3272,7 @@ We can see that we got the same content back, which we had earlier written to th
 
 .. note::
 
-   You may need to install xclip or xsel (with gtk, PyQt5, PyQt4 or qtpy) on Linux to use these methods.
+   You may need to install xclip or xsel (with PyQt5, PyQt4 or qtpy) on Linux to use these methods.
 
 .. _io.pickle:
 
diff --git a/doc/source/whatsnew/v0.25.0.rst b/doc/source/whatsnew/v0.25.0.rst
index 89a9da4a73b35..65ef332b4122c 100644
--- a/doc/source/whatsnew/v0.25.0.rst
+++ b/doc/source/whatsnew/v0.25.0.rst
@@ -334,6 +334,7 @@ Other API Changes
 - The ``arg`` argument in :meth:`pandas.core.groupby.DataFrameGroupBy.agg` has been renamed to ``func`` (:issue:`26089`)
 - The ``arg`` argument in :meth:`pandas.core.window._Window.aggregate` has been renamed to ``func`` (:issue:`26372`)
 - Most Pandas classes had a ``__bytes__`` method, which was used for getting a python2-style bytestring representation of the object. This method has been removed as a part of dropping Python2 (:issue:`26447`)
+- Removed support of gtk package for clipboards (:issue:`26563`)
 
 .. _whatsnew_0250.deprecations:
 
diff --git a/pandas/core/generic.py b/pandas/core/generic.py
index 87db069d94893..9c671b2d3bb34 100644
--- a/pandas/core/generic.py
+++ b/pandas/core/generic.py
@@ -2679,7 +2679,7 @@ def to_clipboard(self, excel=True, sep=None, **kwargs):
         -----
         Requirements for your platform.
 
-          - Linux : `xclip`, or `xsel` (with `gtk` or `PyQt4` modules)
+          - Linux : `xclip`, or `xsel` (with `PyQt4` modules)
           - Windows : none
           - OS X : none
 
diff --git a/pandas/io/clipboard/__init__.py b/pandas/io/clipboard/__init__.py
index b76a843e3e7f2..2063978c76c5a 100644
--- a/pandas/io/clipboard/__init__.py
+++ b/pandas/io/clipboard/__init__.py
@@ -18,21 +18,19 @@
 On Linux, install xclip or xsel via package manager. For example, in Debian:
 sudo apt-get install xclip
 
-Otherwise on Linux, you will need the gtk, qtpy or PyQt modules installed.
+Otherwise on Linux, you will need the qtpy or PyQt modules installed.
 qtpy also requires a python-qt-bindings module: PyQt4, PyQt5, PySide, PySide2
 
-gtk and PyQt4 modules are not available for Python 3,
-and this module does not work with PyGObject yet.
+This module does not work with PyGObject yet.
 """
 __version__ = '1.5.27'
 
 import platform
 import os
 import subprocess
-from .clipboards import (init_osx_clipboard,
-                         init_gtk_clipboard, init_qt_clipboard,
-                         init_xclip_clipboard, init_xsel_clipboard,
-                         init_klipper_clipboard, init_no_clipboard)
+from .clipboards import (
+    init_osx_clipboard, init_qt_clipboard, init_xclip_clipboard,
+    init_xsel_clipboard, init_klipper_clipboard, init_no_clipboard)
 from .windows import init_windows_clipboard
 
 # `import qtpy` sys.exit()s if DISPLAY is not in the environment.
@@ -60,14 +58,6 @@ def determine_clipboard():
         return init_osx_clipboard()
     if HAS_DISPLAY:
         # Determine which command/module is installed, if any.
-        try:
-            # Check if gtk is installed
-            import gtk  # noqa
-        except ImportError:
-            pass
-        else:
-            return init_gtk_clipboard()
-
         try:
             # qtpy is a small abstraction layer that lets you write
             # applications using a single api call to either PyQt or PySide
@@ -104,7 +94,6 @@ def set_clipboard(clipboard):
     global copy, paste
 
     clipboard_types = {'osx': init_osx_clipboard,
-                       'gtk': init_gtk_clipboard,
                        'qt': init_qt_clipboard,
                        'xclip': init_xclip_clipboard,
                        'xsel': init_xsel_clipboard,
diff --git a/pandas/io/clipboard/clipboards.py b/pandas/io/clipboard/clipboards.py
index 66e2e35bf0c59..52abdeafb5ecc 100644
--- a/pandas/io/clipboard/clipboards.py
+++ b/pandas/io/clipboard/clipboards.py
@@ -22,22 +22,6 @@ def paste_osx():
     return copy_osx, paste_osx
 
 
-def init_gtk_clipboard():
-    import gtk
-
-    def copy_gtk(text):
-        global cb
-        cb = gtk.Clipboard()
-        cb.set_text(text)
-        cb.store()
-
-    def paste_gtk():
-        clipboardContents = gtk.Clipboard().wait_for_text()
-        return clipboardContents
-
-    return copy_gtk, paste_gtk
-
-
 def init_qt_clipboard():
     # $DISPLAY should exist
 
diff --git a/pandas/io/clipboards.py b/pandas/io/clipboards.py
index be1256edf7afe..dc30285895dd5 100644
--- a/pandas/io/clipboards.py
+++ b/pandas/io/clipboards.py
@@ -91,7 +91,7 @@ def to_clipboard(obj, excel=True, sep=None, **kwargs):  # pragma: no cover
     Notes
     -----
     Requirements for your platform
-      - Linux: xclip, or xsel (with gtk or PyQt4 modules)
+      - Linux: xclip, or xsel (with PyQt4 modules)
       - Windows:
       - OS X:
     """