diff --git a/.travis.yml b/.travis.yml
index e478d71a5c350..405f9a013a330 100644
--- a/.travis.yml
+++ b/.travis.yml
@@ -36,14 +36,6 @@ matrix:
       env:
         - JOB="3.7" ENV_FILE="ci/deps/travis-37.yaml" PATTERN="(not slow and not network)"
 
-    - dist: trusty
-      env:
-        - JOB="2.7" ENV_FILE="ci/deps/travis-27.yaml" PATTERN="(not slow or (single and db))"
-      addons:
-        apt:
-          packages:
-          - python-gtk2
-
     - dist: trusty
       env:
         - JOB="3.6, locale" ENV_FILE="ci/deps/travis-36-locale.yaml" PATTERN="((not slow and not network) or (single and db))" LOCALE_OVERRIDE="zh_CN.UTF-8"
diff --git a/azure-pipelines.yml b/azure-pipelines.yml
index f0567d76659b6..6c30ec641f292 100644
--- a/azure-pipelines.yml
+++ b/azure-pipelines.yml
@@ -10,7 +10,6 @@ jobs:
     name: Linux
     vmImage: ubuntu-16.04
 
-# Windows Python 2.7 needs VC 9.0 installed, handled in the template
 - template: ci/azure/windows.yml
   parameters:
     name: Windows
diff --git a/ci/azure/posix.yml b/ci/azure/posix.yml
index b9e0cd0b9258c..79218d7fd2a9b 100644
--- a/ci/azure/posix.yml
+++ b/ci/azure/posix.yml
@@ -15,18 +15,6 @@ jobs:
           PATTERN: "not slow and not network"
 
       ${{ if eq(parameters.name, 'Linux') }}:
-        py27_np_120:
-          ENV_FILE: ci/deps/azure-27-compat.yaml
-          CONDA_PY: "27"
-          PATTERN: "not slow and not network"
-
-        py27_locale_slow_old_np:
-          ENV_FILE: ci/deps/azure-27-locale.yaml
-          CONDA_PY: "27"
-          PATTERN: "slow"
-          LOCALE_OVERRIDE: "zh_CN.UTF-8"
-          EXTRA_APT: "language-pack-zh-hans"
-
         py36_locale_slow:
           ENV_FILE: ci/deps/azure-36-locale_slow.yaml
           CONDA_PY: "36"
diff --git a/ci/azure/windows.yml b/ci/azure/windows.yml
index cece002024936..13f2442806422 100644
--- a/ci/azure/windows.yml
+++ b/ci/azure/windows.yml
@@ -12,23 +12,12 @@ jobs:
         ENV_FILE: ci/deps/azure-windows-36.yaml
         CONDA_PY: "36"
 
-      py27_np121:
-        ENV_FILE: ci/deps/azure-windows-27.yaml
-        CONDA_PY: "27"
-
   steps:
     - task: CondaEnvironment@1
       inputs:
         updateConda: no
         packageSpecs: ''
 
-    - powershell: |
-        $wc = New-Object net.webclient
-        $wc.Downloadfile("https://download.microsoft.com/download/7/9/6/796EF2E4-801B-4FC4-AB28-B59FBF6D907B/VCForPython27.msi", "VCForPython27.msi")
-        Start-Process "VCForPython27.msi" /qn -Wait
-      displayName: 'Install VC 9.0 only for Python 2.7'
-      condition: eq(variables.CONDA_PY, '27')
-
     - script: |
         ci\\incremental\\setup_conda_environment.cmd
       displayName: 'Before Install'
diff --git a/ci/deps/azure-27-compat.yaml b/ci/deps/azure-27-compat.yaml
deleted file mode 100644
index 8899e22bdf6cf..0000000000000
--- a/ci/deps/azure-27-compat.yaml
+++ /dev/null
@@ -1,26 +0,0 @@
-name: pandas-dev
-channels:
-  - defaults
-  - conda-forge
-dependencies:
-  - bottleneck=1.2.0
-  - cython=0.28.2
-  - jinja2=2.8
-  - numexpr=2.6.1
-  - numpy=1.12.0
-  - openpyxl=2.5.5
-  - pytables=3.4.2
-  - python-dateutil=2.5.0
-  - python=2.7*
-  - pytz=2013b
-  - scipy=0.18.1
-  - xlrd=1.0.0
-  - xlsxwriter=0.5.2
-  - xlwt=0.7.5
-  # universal
-  - pytest
-  - pytest-xdist
-  - pip:
-    - html5lib==1.0b2
-    - beautifulsoup4==4.2.1
-    - hypothesis>=3.58.0
diff --git a/ci/deps/azure-27-locale.yaml b/ci/deps/azure-27-locale.yaml
deleted file mode 100644
index 0846ef5e8264e..0000000000000
--- a/ci/deps/azure-27-locale.yaml
+++ /dev/null
@@ -1,28 +0,0 @@
-name: pandas-dev
-channels:
-  - defaults
-  - conda-forge
-dependencies:
-  - bottleneck=1.2.0
-  - cython=0.28.2
-  - lxml
-  - matplotlib=2.0.0
-  - numpy=1.12.0
-  - openpyxl=2.4.0
-  - python-dateutil
-  - python-blosc
-  - python=2.7
-  - pytz
-  - pytz=2013b
-  - scipy
-  - sqlalchemy=0.8.1
-  - xlrd=1.0.0
-  - xlsxwriter=0.5.2
-  - xlwt=0.7.5
-  # universal
-  - pytest
-  - pytest-xdist
-  - hypothesis>=3.58.0
-  - pip:
-    - html5lib==1.0b2
-    - beautifulsoup4==4.2.1
diff --git a/ci/deps/azure-windows-27.yaml b/ci/deps/azure-windows-27.yaml
deleted file mode 100644
index b1533b071fa74..0000000000000
--- a/ci/deps/azure-windows-27.yaml
+++ /dev/null
@@ -1,31 +0,0 @@
-name: pandas-dev
-channels:
-  - defaults
-  - conda-forge
-dependencies:
-  - beautifulsoup4
-  - bottleneck
-  - dateutil
-  - gcsfs
-  - html5lib
-  - jinja2=2.8
-  - lxml
-  - matplotlib=2.0.1
-  - numexpr
-  - numpy=1.12*
-  - openpyxl
-  - pytables
-  - python=2.7.*
-  - pytz
-  - s3fs
-  - scipy
-  - sqlalchemy
-  - xlrd
-  - xlsxwriter
-  - xlwt
-  # universal
-  - cython>=0.28.2
-  - pytest
-  - pytest-xdist
-  - moto
-  - hypothesis>=3.58.0
diff --git a/ci/deps/travis-27.yaml b/ci/deps/travis-27.yaml
deleted file mode 100644
index 2624797b24fa1..0000000000000
--- a/ci/deps/travis-27.yaml
+++ /dev/null
@@ -1,49 +0,0 @@
-name: pandas-dev
-channels:
-  - defaults
-  - conda-forge
-dependencies:
-  - beautifulsoup4
-  - bottleneck
-  - cython=0.28.2
-  - fastparquet>=0.2.1
-  - gcsfs
-  - html5lib
-  - ipython
-  - jemalloc=4.5.0.post
-  - jinja2=2.8
-  - lxml
-  - matplotlib=2.2.2
-  - mock
-  - nomkl
-  - numexpr
-  - numpy=1.13*
-  - openpyxl=2.4.0
-  - patsy
-  - psycopg2
-  - py
-  - pyarrow=0.9.0
-  - PyCrypto
-  - pymysql=0.6.3
-  - pytables
-  - blosc=1.14.3
-  - python-blosc
-  - python-dateutil=2.5.0
-  - python=2.7*
-  - pytz=2013b
-  - s3fs
-  - scipy
-  - sqlalchemy=0.9.6
-  - xarray=0.9.6
-  - xlrd=1.0.0
-  - xlsxwriter=0.5.2
-  - xlwt=0.7.5
-  # universal
-  - pytest
-  - pytest-xdist
-  - moto==1.3.4
-  - hypothesis>=3.58.0
-  - pip:
-    - backports.lzma
-    - pandas-gbq
-    - pathlib
diff --git a/tox.ini b/tox.ini
index f055251581a93..8f43b9b7dbec5 100644
--- a/tox.ini
+++ b/tox.ini
@@ -4,7 +4,7 @@
 # and then run "tox" from this directory.
 
 [tox]
-envlist = py27, py35, py36
+envlist = py35, py36
 
 [testenv]
 deps =
@@ -43,13 +43,6 @@ commands =
     # tox should provide a preinstall-commands hook.
     pip uninstall pandas -qy
 
-[testenv:py27]
-deps =
-    numpy==1.8.1
-    boto
-    bigquery
-    {[testenv]deps}
-
 [testenv:py35]
 deps =
     numpy==1.10.0