diff --git a/pandas-stubs/core/frame.pyi b/pandas-stubs/core/frame.pyi
index 89d839f9e..6972bb223 100644
--- a/pandas-stubs/core/frame.pyi
+++ b/pandas-stubs/core/frame.pyi
@@ -160,8 +160,8 @@ class _LocIndexerFrame(_LocIndexer):
         | Callable[[DataFrame], IndexType | MaskType | list[HashableT]]
         | list[HashableT]
         | tuple[
-            IndexType | MaskType | list[HashableT] | Hashable,
-            list[HashableT] | slice | Series[bool] | Callable,
+            Iterable[HashableT] | slice | Hashable,
+            list[HashableT] | Series[bool] | slice | Callable,
         ],
     ) -> DataFrame: ...
     @overload
diff --git a/tests/test_frame.py b/tests/test_frame.py
index c0dc5c7f4..e9093de13 100644
--- a/tests/test_frame.py
+++ b/tests/test_frame.py
@@ -2483,3 +2483,9 @@ def test_xs_frame_new() -> None:
     s2 = df.xs("num_wings", axis=1)
     check(assert_type(s1, Union[pd.Series, pd.DataFrame]), pd.DataFrame)
     check(assert_type(s2, Union[pd.Series, pd.DataFrame]), pd.Series)
+
+
+def test_loc_tuple_slice_list() -> None:
+    """Test DataFrame.loc[index, columns]."""
+    foo = pd.DataFrame(np.random.rand(10, 3), columns=["a", "b", "c"])
+    check(assert_type(foo.loc[4:5, ["a", "b"]], pd.DataFrame), pd.DataFrame)