diff --git a/aws_lambda_powertools/event_handler/api_gateway.py b/aws_lambda_powertools/event_handler/api_gateway.py
index 5b91a1583d4..f96b8789308 100644
--- a/aws_lambda_powertools/event_handler/api_gateway.py
+++ b/aws_lambda_powertools/event_handler/api_gateway.py
@@ -40,7 +40,7 @@
 logger = logging.getLogger(__name__)
 
 _DYNAMIC_ROUTE_PATTERN = r"(<\w+>)"
-_SAFE_URI = "-._~()'!*:@,;"  # https://www.ietf.org/rfc/rfc3986.txt
+_SAFE_URI = "-._~()'!*:@,;="  # https://www.ietf.org/rfc/rfc3986.txt
 # API GW/ALB decode non-safe URI chars; we must support them too
 _UNSAFE_URI = "%<> \[\]{}|^"  # noqa: W605
 _NAMED_GROUP_BOUNDARY_PATTERN = rf"(?P\1[{_SAFE_URI}{_UNSAFE_URI}\\w]+)"
diff --git a/tests/functional/event_handler/test_api_gateway.py b/tests/functional/event_handler/test_api_gateway.py
index 76174909b01..61c2f715b18 100644
--- a/tests/functional/event_handler/test_api_gateway.py
+++ b/tests/functional/event_handler/test_api_gateway.py
@@ -909,7 +909,7 @@ def get_network_account(account_id: str, network_id: str):
     [
         pytest.param(123456789, id="num"),
         pytest.param("user@example.com", id="email"),
-        pytest.param("-._~'!*:@,;()", id="safe-rfc3986"),
+        pytest.param("-._~'!*:@,;()=", id="safe-rfc3986"),
         pytest.param("%<>[]{}|^", id="unsafe-rfc3986"),
     ],
 )